CCNB1 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesCCNB; CCNB1
ProductOrderC
Clone#5G6
Entrez GenelD891
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human CCNB1 expressed in E. Coli.
MW60kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Rat
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
1-197
Catalog#: 30124
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Images
Western Blot
Figure 1: Western blot analysis using CCNB1 mouse mAb against Hela (1) and PC-12 (2) cell lysate.
Figure 1: Western blot analysis using CCNB1 mouse mAb against Hela (1) and PC-12 (2) cell lysate.
Immunofluorescence analysis
Figure 2: Immunofluorescence analysis of Hela cells using CCNB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 2: Immunofluorescence analysis of Hela cells using CCNB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 3: Flow cytometric analysis of Hela cells using CCNB1 mouse mAb (green) and negative control (purple).
Figure 3: Flow cytometric analysis of Hela cells using CCNB1 mouse mAb (green) and negative control (purple).
Product Overview
Description

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. (provided by RefSeq) It has higher expression in tumor tissues .

References (references)
References (references)1. Br J Cancer. 2009 Oct 20;101(8):1461-8.
2. Cancer Res. 2010 Feb 1;70(3):1265-74.
3. J Biol Chem. 2010 Jun 4;285(23):17833-45.