CCNB1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. (provided by RefSeq) It has higher expression in tumor tissues .
Product Overview
Entrez GenelD
891
Aliases
CCNB; CCNB1
Clone#
5G6
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Rat
Immunogen
Purified recombinant fragment of human CCNB1 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Br J Cancer. 2009 Oct 20;101(8):1461-8.
2. Cancer Res. 2010 Feb 1;70(3):1265-74.
3. J Biol Chem. 2010 Jun 4;285(23):17833-45.
2. Cancer Res. 2010 Feb 1;70(3):1265-74.
3. J Biol Chem. 2010 Jun 4;285(23):17833-45.
Product Image
Western Blot
Figure 1: Western blot analysis using CCNB1 mouse mAb against Hela (1) and PC-12 (2) cell lysate.
Immunofluorescence analysis
Figure 2: Immunofluorescence analysis of Hela cells using CCNB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 3: Flow cytometric analysis of Hela cells using CCNB1 mouse mAb (green) and negative control (purple).
For Research Use Only. Not for use in diagnostic procedures.