Mouse Monoclonal Antibody to CCL27
Item Information
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Description
This gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene is chemotactic for skin-associated memory T lymphocytes. This cytokine may also play a role in mediating homing of lymphocytes to cutaneous sites. It specifically binds to chemokine receptor 10 (CCR10). Studies of a similar murine protein indicate that these protein-receptor interactions have a pivotal role in T cell-mediated skin inflammation. [provided by RefSeq, Sep 2014]
Product Overview
Entrez GenelD
10850
Aliases
ALP; ILC; CTAK; CTACK; PESKY; ESKINE; SCYA27
Clone#
3B7A5
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human CCL27 (AA: 25-112) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Exp Dermatol. 2018 Feb;27(2):207-210. 2,BMC Cancer. 2018 Jan 2;18(1):9.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CCL27 mAb against human CCL27 (AA: 25-112) recombinant protein. (Expected MW is 36 kDa)
Western Blot
Figure 3:Western blot analysis using CCL27 mAb against HEK293-6e (1) and CCL27 (AA: 25-112)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of HepG2 cells using CCL27 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 5:Flow cytometric analysis of Hela cells using CCL27 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 6:Flow cytometric analysis of A431 cells using CCL27 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using CCL27 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using CCL27 mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 9:Immunofluorescence analysis of Hela cells using CCL27 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.