CBX8 Primary Antibody
Item Information
Catalog #
Size
Price
Description
Chromobox homolog 8 (CBX8), a Polycomb Group protein that interacts with MLL-AF9 and TIP60, plays an essential role in MLL-AF9 transcriptional regulation and leukemogenesis. CBX8, which is part of one of the PRC1 complexes, regulates proliferation of diploid human and mouse fibroblasts through direct binding to the INK4A-ARF locus.
Product Overview
Entrez GenelD
57332
Aliases
PC3; RC1
Clone#
9C3D4
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CBX8 (AA: 17-222) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Cancer Cell. 2011 Nov 15;20(5):563-75.
2.EMBO J. 2007 Mar 21;26(6):1637-48.
2.EMBO J. 2007 Mar 21;26(6):1637-48.
Product Image
Western Blot
Figure 1: Western blot analysis using CBX8 mAb against human CBX8 recombinant protein. (Expected MW is 49.5 kDa)
Western Blot
Figure 2: Western blot analysis using CBX8 mAb against HEK293 (1) and CBX8 (AA: 17-222)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of HeLa cells using CBX8 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.