CBX2 Primary Antibody
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Description
This gene encodes a component of the polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. Disruption of this gene in mice results in male-to-female gonadal sex reversal. Mutations in this gene are also associated with gonadal dysgenesis in humans. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
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Elisa

Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot

Figure 2:Western blot analysis using CBX2 mAb against human CBX2 (AA: 402-525) recombinant protein. (Expected MW is 38.2 kDa)
Western Blot

Figure 3:Western blot analysis using CBX2 mAb against HEK293 (1) and CBX2 (AA: 402-525)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot

Figure 4:Western blot analysis using CBX2 mouse mAb against HUVEC (1), HEK293 (2), Hela (3), NIH/3T3 (4), and A431 (5) cell lysate.
Immunofluorescence analysis

Figure 5:Immunofluorescence analysis of MCF-7 cells using CBX2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric

Figure 6:Flow cytometric analysis of HeLa cells using CBX2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis

Figure 7:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CBX2 mouse mAb with DAB staining.
Immunohistochemical analysis

Figure 8:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CBX2 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.