CBX2 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a component of the polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. Disruption of this gene in mice results in male-to-female gonadal sex reversal. Mutations in this gene are also associated with gonadal dysgenesis in humans. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Product Overview
Entrez GenelD
84733
Aliases
M33; CDCA6; SRXY5
Clone#
4C11B10
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse
Immunogen
Purified recombinant fragment of human CBX2 (AA: 402-525) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Br J Cancer. 2014 Oct 14;111(8):1663-72.
2.Fertil Steril. 2013 Mar 1;99(3):819-826.
2.Fertil Steril. 2013 Mar 1;99(3):819-826.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using CBX2 mAb against human CBX2 (AA: 402-525) recombinant protein. (Expected MW is 38.2 kDa)
Western Blot
Figure 3:Western blot analysis using CBX2 mAb against HEK293 (1) and CBX2 (AA: 402-525)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using CBX2 mouse mAb against HUVEC (1), HEK293 (2), Hela (3), NIH/3T3 (4), and A431 (5) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of MCF-7 cells using CBX2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 6:Flow cytometric analysis of HeLa cells using CBX2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CBX2 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CBX2 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.