CBX2 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesM33; CDCA6; SRXY5
ProductOrderC
Clone#4C11B10
Entrez GenelD84733
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human CBX2 (AA: 402-525) expressed in E. Coli.
MW56kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Mouse
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
402-525
Catalog#: 30881
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Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using CBX2 mAb against human CBX2 (AA: 402-525) recombinant protein. (Expected MW is 38.2 kDa)
Figure 2:Western blot analysis using CBX2 mAb against human CBX2 (AA: 402-525) recombinant protein. (Expected MW is 38.2 kDa)
Western Blot
Figure 3:Western blot analysis using CBX2 mAb against HEK293 (1) and CBX2 (AA: 402-525)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using CBX2 mAb against HEK293 (1) and CBX2 (AA: 402-525)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using CBX2 mouse mAb against HUVEC (1), HEK293 (2), Hela (3), NIH/3T3 (4), and A431 (5) cell lysate.
Figure 4:Western blot analysis using CBX2 mouse mAb against HUVEC (1), HEK293 (2), Hela (3), NIH/3T3 (4), and A431 (5) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of MCF-7 cells using CBX2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of MCF-7 cells using CBX2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 6:Flow cytometric analysis of HeLa cells using CBX2 mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of HeLa cells using CBX2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CBX2 mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CBX2 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CBX2 mouse mAb with DAB staining.
Figure 8:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CBX2 mouse mAb with DAB staining.
Product Overview
DescriptionThis gene encodes a component of the polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. Disruption of this gene in mice results in male-to-female gonadal sex reversal. Mutations in this gene are also associated with gonadal dysgenesis in humans. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
References (references)
References (references)1.Br J Cancer. 2014 Oct 14;111(8):1663-72.
2.Fertil Steril. 2013 Mar 1;99(3):819-826.