CASP3 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesCPP32; SCA-1; CPP32B
ProductOrderC
Clone#3D4D7
Entrez GenelD836
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human CASP3 (AA: 29-175) expressed in E. Coli.
MW31.6kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
29-175
Catalog#: 30759
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Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using CASP3 mAb against human CASP3 (AA: 29-175) recombinant protein. (Expected MW is 19.5 kDa)
Figure 2:Western blot analysis using CASP3 mAb against human CASP3 (AA: 29-175) recombinant protein. (Expected MW is 19.5 kDa)
Flow cytometric
Figure 4:Flow cytometric analysis of Hela cells using CASP3 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of Hela cells using CASP3 mouse mAb (green) and negative control (red).
Product Overview
Description

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

References (references)
References (references)Cell Death Dis. 2013 Jul 11;4:e725.
PLoS One. 2013 May 2;8(5):e62303.