CASP3 Primary Antibody

Item Information
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Price
Description

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

Product Overview
Entrez GenelD
836
Aliases
CPP32; SCA-1; CPP32B
Clone#
3D4D10
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse, Rat
Immunogen
Purified recombinant fragment of human CASP3 (AA: 29-175) expressed in E. Coli.
Formulation
Purified antibody from tissue culture in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ELISA
1/10000
References
Cell Death Dis. 2013 Jul 11;4:e725.
PLoS One. 2013 May 2;8(5):e62303.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using CASP3 mAb against human CASP3 (AA: 29-175) recombinant protein. (Expected MW is *** kDa)
Figure 2:Western blot analysis using CASP3 mAb against human CASP3 (AA: 29-175) recombinant protein. (Expected MW is *** kDa)
Western Blot
Figure 3:Western blot analysis using CASP3 mouse mAb against Hela (1), Jurkat (2), HepG2 (3), BCL-1 (4), C6 (5), SK-Br-3 (6), NIH/3T3 (7) and A549 (8) cell lysate.
Figure 3:Western blot analysis using CASP3 mouse mAb against Hela (1), Jurkat (2), HepG2 (3), BCL-1 (4), C6 (5), SK-Br-3 (6), NIH/3T3 (7) and A549 (8) cell lysate.
For Research Use Only. Not for use in diagnostic procedures.