CAPN1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
Product Overview
Entrez GenelD
823
Aliases
CANP; muCL; CANP1; SPG76; CANPL1; muCANP
Clone#
1E6G9
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CAPN1 (AA: 501-714) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Biomed Res Int. 2017;2017:9290425.
2.Histopathology. 2016 Jun;68(7):1021-9.
2.Histopathology. 2016 Jun;68(7):1021-9.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CAPN1 mAb against human CAPN1 (AA: 501-714) recombinant protein. (Expected MW is *** kDa)
Western Blot
Figure 3:Western blot analysis using CAPN1 mAb against HEK293 (1) and CAPN1 (AA: 501-714)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of Hela cells using CAPN1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.