CALD1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
Product Overview
Entrez GenelD
800
Aliases
CDM; HCAD; LCAD; H-CAD; L-CAD; NAG22
Clone#
6F8D2
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human CALD1 (AA: 26-207) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4?; -20? for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Biol Reprod. 2013 May 16;88(5):122. 2.J Diabetes Complications. 2011 Mar-Apr;25(2):114-21.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CALD1 mAb against human CALD1 (AA: 26-207) recombinant protein. (Expected MW is 47.6 kDa)
Western Blot
Figure 3:Western blot analysis using CALD1 mAb against HEK293 (1) and CALD1 (AA: 26-207)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometric
Figure 4:Flow cytometric analysis of Hela cells using CALD1 mouse mAb (green) and negative control (red).
Immunohistochemical Analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using CALD1 mouse mAb with DAB staining.
Immunohistochemical Analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CALD1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.