Mouse Monoclonal Antibody to C5AR2
Item Information
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Price
Description
This gene encodes a G-protein coupled receptor 1 family member involved in the complement system of the innate immune response. Unlike classical G-protein coupled receptors, the encoded protein does not associate with intracellular G-proteins. It may instead modulate signal transduction through the beta-arrestin pathway, and may alternatively act as a decoy receptor. This gene may be involved in coronary artery disease and in the pathogenesis of sepsis. Alternative splicing results in multiple transcript variants.
Product Overview
Entrez GenelD
27202
Aliases
C5L2; GPF77; GPR77
Clone#
6D4A4
Host / Isotype
Mouse / IgG2b
Immunogen
Purified recombinant fragment of human C5AR2 (AA: extra mix) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Oncotarget. 2017 Jan 31;8(5):8590-8596.2,J Dent Res. 2017 Jan;96(1):92-99.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using C5AR2 mAb against human C5AR2 (AA: extra mix) recombinant protein. (Expected MW is 25 kDa)
Western Blot
Figure 3:Western blot analysis using C5AR2 mAb against HEK293-6e (1) and human C5AR2 (AA: extra mix)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using C5AR2 mouse mAb against K562 (1),THP-1 (2), and MOLT4 (3) cell lysate.
Flow cytometric analysis
Figure 5:Flow cytometric analysis of MOLT4 cells using C5AR2 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 6:Flow cytometric analysis of HL-60 cells using C5AR2 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 7:Flow cytometric analysis of RAW264.7 cells using C5AR2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded liver cancer tissues using C5AR2 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.