C3AR1
| Item Information | ||
|---|---|---|
| Catalog # | Size/Concentration | Price |
| Specification | |
|---|---|
| Aliases | AZ3B; C3AR; HNFAG09 |
| Clone# | 3B5E1 |
| Entrez GenelD | 719 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Host | Mouse |
| Isotype | Mouse IgG1 |
| Immunogen | Purified recombinant fragment of human C3AR1 (AA: 161-340) expressed in E. Coli. |
| MW | 53.8kDa |
| Species Reactivity | Human |
| Application | |
|---|---|
| ELISA | 1/10000 |
| FCM (Flow Cytometry) | 1/200 - 1/400 |
| WB (Western Blot) | 1/500 - 1/2000 |
| Sequence | |
|---|---|
| extra(161-340) | |
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using C3AR1 mAb against human C3AR1 (AA: 161-340) recombinant protein. (Expected MW is 46.2 kDa)
Western Blot
Figure 3:Western blot analysis using C3AR1 mAb against HEK293-6e (1) and C3AR1 (AA: 161-340)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunofluorescence analysis
Figure 4:Flow cytometric analysis of THP-1 cells using C3AR1 mouse mAb (green) and negative control (red).