C3AR1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
C3a is an anaphylatoxin released during activation of the complement system. The protein encoded by this gene is an orphan G protein-coupled receptor for C3a. Binding of C3a by the encoded receptor activates chemotaxis, granule enzyme release, superoxide anion production, and bacterial opsonization.
Product Overview
Entrez GenelD
719
Aliases
AZ3B; C3AR; HNFAG09
Clone#
3B5E1
Host / Isotype
Mouse / Mouse IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human C3AR1 (AA: 161-340) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Cell Rep. 2019 Sep 3;28(10):2567-2580.e6.
2.ESC Heart Fail. 2018 Oct;5(5):846-857.
2.ESC Heart Fail. 2018 Oct;5(5):846-857.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using C3AR1 mAb against human C3AR1 (AA: 161-340) recombinant protein. (Expected MW is 46.2 kDa)
Western Blot
Figure 3:Western blot analysis using C3AR1 mAb against HEK293-6e (1) and C3AR1 (AA: 161-340)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunofluorescence analysis
Figure 4:Flow cytometric analysis of THP-1 cells using C3AR1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.