C1QA Primary Antibody
Item Information
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Description
This gene encodes the A-chain polypeptide of serum complement subcomponent C1q, which associates with C1r and C1s to yield the first component of the serum complement system. C1q deficiency is associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains which include 6 A-chains, 6 B-chains, and 6 C-chains. Each chain contains an N-terminal collagen-like region and a C-terminal C1q globular domain. Alternate splicing results in multiple transcript variants.
Product Overview
Entrez GenelD
712
Clone#
1C9G9
Host / Isotype
Mouse / Mouse IgG2b
Immunogen
Purified recombinant fragment of human C1QA (AA: 96-245) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200-1/400
ELISA
1/10000
References
1.Front Immunol. 2018 Oct 23;9:2427. 2.Mol Immunol. 2016 Oct;78:164-170.
Product Image
ELISA
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
Figure 2: Western blot analysis using C1QA mAb against human C1QA (AA: 96-245) recombinant protein. (Expected MW is 19.4 kDa)
WESTERN BLOT
Figure 3: Western blot analysis using C1QA mAb against HEK293-6e (1) and C1QA (AA: 96-245)-hIgGFc transfected HEK293-6e (2) cell lysate.
FLOW CYTOMETRY
Figure 4: Flow cytometric analysis of Hela cells using C1QA mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.