C-CBL Primary Antibody
Item Information
Catalog #
Size
Price
Description
The cbl oncogene was first identified as part of a transforming retrovirus which induces mouse pre-B and pro-B cell lymphomas. As an adaptor protein for receptor protein-tyrosine kinases, it positively regulates receptor protein-tyrosine kinase ubiquitination in a manner dependent upon its variant SH2 and RING finger domains. Ubiquitination of receptor protein-tyrosine kinases terminates signaling by marking active receptors for degradation.
Product Overview
Entrez GenelD
867
Aliases
CBL; CBL2; NSLL; C-CBL; RNF55
Clone#
3B12
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse, Rat
Immunogen
Purified recombinant fragment of human C-CBL expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Blood. 2009 Aug 27;114(9):1859-63.
2. Cell Res. 2009 Aug;19(8):950-61.
3. Nature. 2009 Aug 13;460(7257):904-8.
2. Cell Res. 2009 Aug;19(8):950-61.
3. Nature. 2009 Aug 13;460(7257):904-8.
Product Image
Western Blot
Figure 1: Western blot analysis using C-CBL mAb against human C-CBL (AA: 684-865) recombinant protein. (Expected MW is 44.9 kDa)
Western Blot
Figure 2: Western blot analysis using C-CBL mouse mAb against RAJI (1), RAW264.7 (2), K562 (3), SKBR-3 (4), 3T3-L1 (5), THP-1 (6) and PC-12 (7) cell lysate.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using C-CBL mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using C-CBL mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 5: Immunofluorescence analysis of Hela cells using C-CBL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 6: Flow cytometric analysis of MCF-7 cells using C-CBL mouse mAb (blue) and negative control (red).
Elisa
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
For Research Use Only. Not for use in diagnostic procedures.