BUB1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants.
Product Overview
Entrez GenelD
699
Aliases
BUB1A; BUB1L; Hbub1
Clone#
1A11C7
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human BUB1 (AA: 1-130) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Sci Signal. 2015 Jan 6;8(358):ra1.
2.Horm Cancer. 2013 Apr;4(2):92-102.
2.Horm Cancer. 2013 Apr;4(2):92-102.
Product Image
Western Blot
Figure 1:Western blot analysis using BUB1 mAb against human BUB1 (AA: 1-130) recombinant protein. (Expected MW is 41.3 kDa)
Western Blot
Figure 2:Western blot analysis using BUB1 mAb against HEK293 (1) and BUB1 (AA: 1-130)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 3:Flow cytometric analysis of Hela cells using BUB1 mouse mAb (green) and negative control (red).
Elisa
Figure 4:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
For Research Use Only. Not for use in diagnostic procedures.