BTN1A1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
Butyrophilin is the major protein associated with fat droplets in the milk. It is a member of the immunoglobulin superfamily. It may have a cell surface receptor function. The human butyrophilin gene is localized in the major histocompatibility complex (MHC) class I region of 6p and may have arisen relatively recently in evolution by the shuffling of exons between 2 ancestral gene families
Product Overview
Entrez GenelD
696
Aliases
BT; BTN; BTN1
Clone#
5B12B9
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human, Mouse, Rat
Immunogen
Purified recombinant fragment of human BTN1A1 (AA: extra 27-242) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.PLoS One. 2011;6(9):e24432.
2.J Biol Chem. 2009 Aug 14;284(33):22444-56.
2.J Biol Chem. 2009 Aug 14;284(33):22444-56.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using BTN1A1 mAb against human BTN1A1 (AA: extra 27-242) recombinant protein. (Expected MW is 50 kDa)
Western Blot
Figure 3:Western blot analysis using BTN1A1 mAb against HEK293 (1) and BTN1A1 (AA: extra 27-242)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using BTN1A1 mouse mAb against HepG2 (1), MCF-7 (2), SK-BR-3 (3), NIH/3T3 (4), and C6 (5) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of K562 cells using BTN1A1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.