BTLA Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene encodes a member of the immunoglobulin superfamily. The encoded protein contains a single immunoglobulin (Ig) domain and is a receptor that relays inhibitory signals to suppress the immune response. Alternative splicing results in multiple transcript variants. Polymorphisms in this gene have been associated with an increased risk of rheumatoid arthritis.

Product Overview

Entrez GenelD

151888

Aliases

BTLA1; CD272

Clone#

7B8B4

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human BTLA (AA: extra 31-157) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/100 - 1/500

ELISA

1/10000

References

1.Blood. 2013 Aug 8;122(6):922-31.
2.Breast Cancer Res Treat. 2010 Feb;120(1):195-202.

Product Image

Elisa

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using BTLA mAb against human BTLA (AA: extra 31-157) recombinant protein. (Expected MW is 41 kDa)

Western Blot

Figure 3:Western blot analysis using BTLA mAb against HEK293 (1) and BTLA (AA: extra 31-157)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Figure 4:Immunofluorescence analysis of Hela cells using BTLA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

For Research Use Only. Not for use in diagnostic procedures.