BRD2 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a transcriptional regulator that belongs to the BET (bromodomains and extra terminal domain) family of proteins. This protein associates with transcription complexes and with acetylated chromatin during mitosis, and it selectively binds to the acetylated lysine-12 residue of histone H4 via its two bromodomains. The gene maps to the major histocompatability complex (MHC) class II region on chromosome 6p21.3, but sequence comparison suggests that the protein is not involved in the immune response. This gene has been implicated in juvenile myoclonic epilepsy, a common form of epilepsy that becomes apparent in adolescence. Multiple alternatively spliced variants have been described for this gene.
Product Overview
Entrez GenelD
6046
Aliases
FSH; NAT; RNF3; FSRG1; RING3; D6S113E; O27.1.1
Clone#
7C1B10
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human, Rat
Immunogen
Purified recombinant fragment of human BRD2 (AA: 227-364) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Mol Biol Cell. 2013 Nov;24(22):3557-68.
2.J Biol Chem. 2010 Mar 5;285(10):7610-8.
2.J Biol Chem. 2010 Mar 5;285(10):7610-8.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using BRD2 mAb against human BRD2 (AA: 227-364) recombinant protein. (Expected MW is 40.6 kDa)
Western Blot
Figure 3:Western blot analysis using BRD2 mAb against HEK293 (1) and BRD2 (AA: 227-364)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using BRD2 mouse mAb against C6 (1) and Hela (2) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of Hela cells using BRD2 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.