Mouse Monoclonal Antibody to BRAF
This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017]
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/44369b8da414a76ee927b549912fe721d875b09b8b925b5c7079f14f967b6571.jpg?v=1666705752)
![Figure 2:Western blot analysis using BRAF mAb against human BRAF (AA: 299-447) recombinant protein. (Expected MW is 46kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/71cd2c9df7111b9cd24faec75dff028369818af33b81d2f5cf0ac6404a8521d5.jpg?v=1666705752)
![Figure 3:Western blot analysis using BRAF mouse mAb against Hela (1), HT-29 (2), MOLT4 (3), T47D (4), HePG2 (5), NIH/3T3 (6), PC-12 (7), and COS-7 (8) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/12e930ce4ceb785125fcfdff2392fa04702e3f4d455982b6c742c300b81819f2.jpg?v=1666705752)
![Figure 4:Flow cytometric analysis of BEL-7402 cells using BRAF mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/aa88e8a041fe53b6808075d2dc138f91b9ed12ada1f099d95dbe1ba69762288a.jpg?v=1666705752)
![Figure 5:Flow cytometric analysis of Hela cells using BRAF mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/60c43c3757d8b3ae28d8fd1cedc2c579de3174640cdd25799cfbd41f89c0db25.jpg?v=1666705752)
![Figure 6:Flow cytometric analysis of HepG2 cells using BRAF mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/de0afb565d7c7d311e00bc15f3506cbcff1529d6fa6ff50b727d5edfef8a3dfe.jpg?v=1666705752)
![Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using BRAF mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/e1c9cd46bb00a318f53c4dec99ef684c2d6a78f1c82f78ee1528428a0bfcabf4.jpg?v=1666705752)
![Figure 8:Immunohistochemical analysis of paraffin-embedded cerebellar tissues using BRAF mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/749e372b6286f121d3e53d107716860622439b6b0e3e723b0f0dbf5ee787b934.jpg?v=1666705752)