Mouse Monoclonal Antibody to BRAF

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Description

This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017]

Product Overview
Entrez GenelD
673
Aliases
NS7; B-raf; BRAF1; RAFB1; B-RAF1
Clone#
3B8B12
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human BRAF (AA: 299-447) expressed in HEK293-6e.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Proc Natl Acad Sci U S A. 2020 Dec 8;117(49):31105-31113. 2,Sci Rep. 2020 Oct 9;10(1):16943.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using BRAF mAb against human BRAF (AA: 299-447) recombinant protein. (Expected MW is 46kDa)
Figure 2:Western blot analysis using BRAF mAb against human BRAF (AA: 299-447) recombinant protein. (Expected MW is 46kDa)
Western Blot
Figure 3:Western blot analysis using BRAF mouse mAb against Hela (1), HT-29 (2), MOLT4 (3), T47D (4), HePG2 (5), NIH/3T3 (6), PC-12 (7), and COS-7 (8) cell lysate.
Figure 3:Western blot analysis using BRAF mouse mAb against Hela (1), HT-29 (2), MOLT4 (3), T47D (4), HePG2 (5), NIH/3T3 (6), PC-12 (7), and COS-7 (8) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of BEL-7402 cells using BRAF mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of BEL-7402 cells using BRAF mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 5:Flow cytometric analysis of Hela cells using BRAF mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of Hela cells using BRAF mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 6:Flow cytometric analysis of HepG2 cells using BRAF mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of HepG2 cells using BRAF mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using BRAF mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using BRAF mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded cerebellar tissues using BRAF mouse mAb with DAB staining.
Figure 8:Immunohistochemical analysis of paraffin-embedded cerebellar tissues using BRAF mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.