Mouse Monoclonal Antibody to BRAF
This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017]
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/d76ab977c8a0d77c12ba0ba4899e45cff9e4d749d858d7107c2a5d18b97b337c.jpg?v=1666705749)
![Figure 2:Western blot analysis using BRAF mAb against human BRAF (AA: 299-447) recombinant protein. (Expected MW is 46kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/d9de794b36578debe86c136d3bf169226cc98c2cad497eb01dee4e3a7fac6367.jpg?v=1666705749)
![Figure 3:Western blot analysis using BRAF mouse mAb against Hela (1), HT-29 (2), MOLT4 (3) T47D (4) HePG2 (5) HL-60 (6) and PC-12 (7) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/42e6b9eed34eaae177ed2baa3d420cc63ecf8798b7b64256a29428711339d029.jpg?v=1666705749)
![Figure 4:Flow cytometric analysis of BEL-7402 cells using BRAF mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/0477fa5257a221a568585e9f21f3693fccc7a0f0e7e18b8edc7ce02158cb3aee.jpg?v=1666705749)
![Figure 5:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using BRAF mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/b892e564930856899b8e3dc2ac16330564492339d3c3e8c4510e057d8ad1bdbc.jpg?v=1666705749)
![Figure 6:Immunohistochemical analysis of paraffin-embedded cerebellar tissues using BRAF mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/6b858198e46efc25a4e1a9f964024ab2405b3fdc7c53396b5dfc221e0f2746b1.jpg?v=1666705749)