BMPR1A Primary Antibody
Item Information
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Size
Price
Description
The bone morphogenetic protein (BMP) receptors are a family of transmembrane serine/threonine kinases that include the type I receptors BMPR1A and BMPR1B and the type II receptor BMPR2. These receptors are also closely related to the activin receptors, ACVR1 and ACVR2. The ligands of these receptors are members of the TGF-beta superfamily. TGF-betas and activins transduce their signals through the formation of heteromeric complexes with 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding.
Product Overview
Entrez GenelD
657
Aliases
ALK3; SKR5; CD292; ACVRLK3; 10q23del
Clone#
4B7B2
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse
Immunogen
Purified recombinant fragment of human BMPR1A (AA: 179-378 ) expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 May 1;67(Pt 5):551-5.
2.Gastroenterology. 2011 Jul;141(1):e23-6.
2.Gastroenterology. 2011 Jul;141(1):e23-6.
Product Image
Western Blot
Figure 1: Western blot analysis using BMPR1A mAb against human BMPR1A recombinant protein. (Expected MW is 48.1 kDa)
Western Blot
Figure 2: Western blot analysis using BMPR1A mAb against HEK293 (1) and BMPR1A (AA: 179-378)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using BMPR1A mouse mAb against PC-3 (1), K562 (2) cell lysate, and Mouse liver (3) tissue lysate.
Flow cytometric
Figure 4: Flow cytometric analysis of HeLa cells using BMPR1A mouse mAb (green) and negative control (purple).
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using BMPR1A mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded kidney tissues using BMPR1A mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.