BMP4 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein.
Product Overview
Entrez GenelD
652
Aliases
ZYME; BMP2B; OFC11; BMP2B1; MCOPS6
Clone#
3C11C7
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Rat
Immunogen
Purified recombinant fragment of human BMP4 (AA: 277-408) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/100 - 1/500
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Cancer Invest. 2013 Oct;31(8):555-62.
2.Eur J Oral Sci. 2013 Aug;121(4):313-8.
2.Eur J Oral Sci. 2013 Aug;121(4):313-8.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using BMP4 mAb against human BMP4 (AA: 277-408) recombinant protein. (Expected MW is 41 kDa)
Western Blot
Figure 3:Western blot analysis using BMP4 mAb against HEK293 (1) and BMP4 (AA: 277-408)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using BMP4 mouse mAb against A549 (1), HepG2 (2), and C6 (3) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using BMP4 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunofluorescence analysis
Figure 6:Immunofluorescence analysis of Hela cells using BMP4 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 7:Flow cytometric analysis of Hela cells using BMP4 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.