BMP2 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene belongs to the transforming growth factor-beta (TGFB) superfamily. The encoded protein acts as a disulfide-linked homodimer and induces bone and cartilage formation.
Product Overview
Entrez GenelD
650
Aliases
BDA2; BMP2A
Clone#
9E10G12
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human BMP2 (AA: 283-396) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Tissue Eng Part A. 2013 Dec;19(23-24):2664-73.
2.BMC Biol. 2012 Apr 30;10:37.
2.BMC Biol. 2012 Apr 30;10:37.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using BMP2 mAb against human BMP2 (AA: 283-396) recombinant protein. (Expected MW is 38.7 kDa)
Western Blot
Figure 3:Western blot analysis using BMP2 mAb against HEK293 (1) and BMP2 (AA: 283-396)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using BMP2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of MCF-7 cells using BMP2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 6:Flow cytometric analysis of Hela cells using BMP2 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.