BMI1 Primary Antibody
| Item Information | ||
|---|---|---|
| Catalog # | Size/Concentration | Price |
| Specification | |
|---|---|
| Aliases | PCGF4; RNF51; MGC12685; FLVI2/BMI1 |
| ProductOrder | B |
| Clone# | 3E3 |
| Entrez GenelD | 648 |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Purified recombinant fragment of human BMI1 expressed in E. Coli. |
| MW | 37kDa |
| Shipping Information | This product will ship in a box containing blue ice at a temperature of 4°C. Learn More |
| Species Reactivity | Human |
| Application | |
|---|---|
| ELISA | 1/10000 |
| ICC (Immunocytochemistry) | 1/200 - 1/1000 |
| IHC_P(Immunohistochemistry) | 1/200 - 1/1000 |
| FCM (Flow Cytometry) | 1/200 - 1/400 |
| WB (Western Blot) | 1/500 - 1/2000 |
| Sequence | |
|---|---|
| 1-326 | |
Western Blot
Figure 1: Western blot analysis using BMI1 mAb against human BMI1 (AA: 1-326) recombinant protein. (Expected MW is 74 kDa)
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using BMI1 mouse mAb with DAB staining.
Western Blot
Figure 2: Western blot analysis using BMI1 mAb against HEK293 (1) and BMI1-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using BMI1 mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 4: Immunofluorescence analysis of Hela cells using BMI1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 5: Flow cytometric analysis of NIH/3T3 cells using BMI1 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);