BLNK Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesAGM4; BASH; LY57; SLP65; BLNK-S; SLP-65; MGC111051
ProductOrderB
Clone#5G9
Entrez GenelD29760
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human BLNK expressed in E. Coli.
MW68kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Mouse
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
34-216
Catalog#: 30232
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Images
Western Blot
Figure 1: Western blot analysis using BLNK mAb against human BLNK (AA: 34-216) recombinant protein. (Expected MW is 60 kDa)
Figure 1: Western blot analysis using BLNK mAb against human BLNK (AA: 34-216) recombinant protein. (Expected MW is 60 kDa)
Western Blot
Figure 2: Western blot analysis using BLNK mouse mAb against NIH/3T3 (1) and BCBL-1 (2) cell lysate.
Figure 2: Western blot analysis using BLNK mouse mAb against NIH/3T3 (1) and BCBL-1 (2) cell lysate.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using BLNK mouse mAb with DAB staining.
Figure 3: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using BLNK mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 4: Immunofluorescence analysis of HepG2 cells using BLNK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 4: Immunofluorescence analysis of HepG2 cells using BLNK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 5: Flow cytometric analysis of NIH/3T3 cells using BLNK mouse mAb (green) and negative control (purple).
Figure 5: Flow cytometric analysis of NIH/3T3 cells using BLNK mouse mAb (green) and negative control (purple).
Elisa
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Product Overview
Description

This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

References (references)
References (references)1. J Biol Chem. 2009 Apr 10;284(15):9804-13.
2. Cancer Sci. 2008 Dec;99(12):2444-54.