BLK Primary Antibody
|Aliases||MODY11; MGC10442; BLK|
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Immunogen||Purified recombinant fragment of human BLK expressed in E. Coli.|
|Shipping Information||This product will ship in a box containing blue ice at a temperature of 4°C. Learn More|
|ICC (Immunocytochemistry)||1/200 - 1/1000|
|FCM (Flow Cytometry)||1/200 - 1/400|
|WB (Western Blot)||1/500 - 1/2000|
Contact us to order
Figure 1: Western blot analysis using BLK mAb against HEK293 (1) and BLK(AA: 2-200)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Immunofluorescence analysis of Hela cells using BLK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 3: Flow cytometric analysis of HL-60 cells using BLK mouse mAb (green) and negative control (purple).
Blk is a Src family protein tyrosine kinase expressed in all stages of B cell development . Activation of B cells by various ligands is accompanied by activation of Blk . It has been suggested that Blk is involved in the control of B cell differentiation and proliferation . Blk transcripts have also been detected in human thymocytes, but not in mature T cells, implicating that Blk may play an important role in thymopoiesis . Blk function may be redundant, however, as mice that do not express Blk are not impaired with respect to B cell development and immune response .
|References (references)||1. N Engl J Med. 2008 Feb 28;358(9):900-9. |
2. Genes Immun. 2009 Apr;10(3):219-26.
3. Proc Natl Acad Sci U S A. 2009 Aug 25;106(34):14460-5.