BLK Primary Antibody
Item Information
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Description
Blk is a Src family protein tyrosine kinase expressed in all stages of B cell development . Activation of B cells by various ligands is accompanied by activation of Blk . It has been suggested that Blk is involved in the control of B cell differentiation and proliferation . Blk transcripts have also been detected in human thymocytes, but not in mature T cells, implicating that Blk may play an important role in thymopoiesis . Blk function may be redundant, however, as mice that do not express Blk are not impaired with respect to B cell development and immune response .
Product Overview
Entrez GenelD
640
Aliases
MODY11; MGC10442; BLK
Clone#
1E6
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human BLK expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. N Engl J Med. 2008 Feb 28;358(9):900-9.
2. Genes Immun. 2009 Apr;10(3):219-26.
3. Proc Natl Acad Sci U S A. 2009 Aug 25;106(34):14460-5.
2. Genes Immun. 2009 Apr;10(3):219-26.
3. Proc Natl Acad Sci U S A. 2009 Aug 25;106(34):14460-5.
Product Image
Western Blot
Figure 1: Western blot analysis using BLK mAb against HEK293 (1) and BLK(AA: 2-200)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 2: Immunofluorescence analysis of Hela cells using BLK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 3: Flow cytometric analysis of HL-60 cells using BLK mouse mAb (green) and negative control (purple).
For Research Use Only. Not for use in diagnostic procedures.