BLK Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesMODY11; MGC10442; BLK
ProductOrderB
Clone#1E6
Entrez GenelD640
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human BLK expressed in E. Coli.
MW58kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
2-200
Catalog#: 30135
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Images
Western Blot
Figure 1: Western blot analysis using BLK mAb against HEK293 (1) and BLK(AA: 2-200)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 1: Western blot analysis using BLK mAb against HEK293 (1) and BLK(AA: 2-200)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 2: Immunofluorescence analysis of Hela cells using BLK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 2: Immunofluorescence analysis of Hela cells using BLK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 3: Flow cytometric analysis of HL-60 cells using BLK mouse mAb (green) and negative control (purple).
Figure 3: Flow cytometric analysis of HL-60 cells using BLK mouse mAb (green) and negative control (purple).
Product Overview
Description

Blk is a Src family protein tyrosine kinase expressed in all stages of B cell development . Activation of B cells by various ligands is accompanied by activation of Blk . It has been suggested that Blk is involved in the control of B cell differentiation and proliferation . Blk transcripts have also been detected in human thymocytes, but not in mature T cells, implicating that Blk may play an important role in thymopoiesis . Blk function may be redundant, however, as mice that do not express Blk are not impaired with respect to B cell development and immune response .

References (references)
References (references)1. N Engl J Med. 2008 Feb 28;358(9):900-9.
2. Genes Immun. 2009 Apr;10(3):219-26.
3. Proc Natl Acad Sci U S A. 2009 Aug 25;106(34):14460-5.