BIRC5 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in adult tissues. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Product Overview
Entrez GenelD
332
Aliases
API4; EPR-1
Clone#
1H5
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human BIRC5 expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. BMC Cancer. 2010 Feb 24;10:65.
2. Int J Cancer. 2009 Oct 15;125(8):1921-5.
2. Int J Cancer. 2009 Oct 15;125(8):1921-5.
Product Image
Western Blot
Figure 1: Western blot analysis using BIRC5 mAb against human BIRC5 (AA: 1-142) recombinant protein. (Expected MW is 42 kDa)
Western Blot
Figure 2: Western blot analysis using BIRC5 mAb against HEK293 (1) and BIRC5 (AA: 1-142)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using BIRC5 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using BIRC5 mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 5: Immunofluorescence analysis of MSCS cells using BIRC5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 6: Flow cytometric analysis of Jurkat cells using BIRC5 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.