beta-Actin Primary Antibody
Item Information
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Size
Price
Description
Beta-actin (PS1TP5-binding protein 1), also known as ACTB, PS1TP5BP1. Entrez Protein NP_001092. It is one of six different actin proteins. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton.Actins are highly conserved proteins that are involved in various types of cell motility, structure, and integrity. Actin is ubiquitously expressed in all eukaryotic cells. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Product Overview
Entrez GenelD
60
Aliases
PS1TP5BP1; ACTB
Clone#
8H10D10
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human, Mouse, Hamster, Rat, Monkey
Immunogen
Synthetic peptide corresponding to amino-terminal residues of human beta-Actin, conjugated to KLH.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Proteomics. 2005 Oct;5(15):3876-84.
2. PLoS Med. 2005 Oct;2(10):e263.
3. Mol Biol Cell. 2005 Nov;16(11):5055-60
4. Nature. 2005 Oct 20;437(7062):1173-8.
2. PLoS Med. 2005 Oct;2(10):e263.
3. Mol Biol Cell. 2005 Nov;16(11):5055-60
4. Nature. 2005 Oct 20;437(7062):1173-8.
Product Image
Western Blot
Figure 1: Western blot analysis using beta-Actin mouse mAb against NIH/3T3 (1), Jurkat (2), Hela (3), CHO (4), PC12 (5), HEK293 (6), COS (7), A549 (8) and MCF-7 (9) cell lysate.
Immunofluorescence analysis
Figure 2: Confocal Immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using beta Actin mouse mAb (red, the secondary Ab is Cy3-Goat anti mouse IgG). Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric
Figure 3: Flow cytometric analysis of MCF-7 cells using beta Actin mouse mAb (right) and negative control (left).
For Research Use Only. Not for use in diagnostic procedures.