BECN1 Primary Antibody

Item Information
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Size
Price
Description

Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 (PubMed 19270693)).(supplied by OMIM) . Tissue specificity: Ubiquitous.

Product Overview
Entrez GenelD
8678
Aliases
ATG6; VPS30; beclin1; BECN1
Clone#
2A4
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human BECN1 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Autophagy. 2008 Oct 1;4(7):947-8.
2. J Clin Invest. 2008 Jun;118(6):2190-9.
Product Image
Western Blot
Figure 1: Western blot analysis using BECN1 mouse mAb against Hela (1), A431 (2), MCF-7 (3), RAJI (4), Jurkat (5) and SKBR-3 (6) cell lysate.
Figure 1: Western blot analysis using BECN1 mouse mAb against Hela (1), A431 (2), MCF-7 (3), RAJI (4), Jurkat (5) and SKBR-3 (6) cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and liver cancer tissues (right) using BECN1 mouse mAb with DAB staining.
Figure 2: Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and liver cancer tissues (right) using BECN1 mouse mAb with DAB staining.
Flow cytometric
Figure 3: Flow cytometric analysis of RAJI cells using BECN1 mouse mAb (green) and negative control (purple).
Figure 3: Flow cytometric analysis of RAJI cells using BECN1 mouse mAb (green) and negative control (purple).
Elisa
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
For Research Use Only. Not for use in diagnostic procedures.