BCL6 Primary Antibody

Item Information

Catalog #

Size

Price

Description

The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of STAT-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene.

Product Overview

Entrez GenelD

604

Aliases

BCL5; LAZ3; BCL6A; ZNF51; ZBTB27

Clone#

2G1B6

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human BCL6 (AA: 147-276) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Cancer Lett. 2015 Sep 1;365(2):190-200.
2.BMC Cancer. 2014 Jun 10;14:418.

Product Image

Elisa

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using BCL6 mAb against human BCL6 (AA: 147-276) recombinant protein. (Expected MW is 40.5 kDa)

Western Blot

Figure 3:Western blot analysis using BCL6 mAb against HEK293 (1) and BCL6 (AA: 147-276)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Figure 4:Immunofluorescence analysis of Hela cells using BCL6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric