BCL2L10 Primary Antibody
Item Information
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Price
Description
The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains conserved BH4, BH1 and BH2 domains. This protein can interact with other members of BCL-2 protein family including BCL2, BCL2L1/BCL-X(L), and BAX. Overexpression of this gene has been shown to suppress cell apoptosis possibly through the prevention of cytochrome C release from the mitochondria, and thus activating caspase-3 activation. The mouse counterpart of this protein is found to interact with Apaf1 and forms a protein complex with Caspase 9, which suggests the involvement of this protein in APAF1 and CASPASE 9 related apoptotic pathway.
Product Overview
Entrez GenelD
10017
Aliases
Boo; Diva; BCL-B; bcl2-L-10
Clone#
8A11G12
Host / Isotype
Mouse / IgG2a
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human BCL2L10 (AA: 31-186) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Hum Reprod. 2013 Mar;28(3):729-39.
2.Oncotarget. 2012 Apr;3(4):490-501.
2.Oncotarget. 2012 Apr;3(4):490-501.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using BCL2L10 mAb against human BCL2L10 (AA: 31-186) recombinant protein. (Expected MW is 43.1 kDa)
Western Blot
Figure 3:Western blot analysis using BCL2L10 mAb against HEK293 (1) and BCL2L10 (AA: 31-186)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of HeLa cells using BCL2L10 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.