Mouse Monoclonal Antibody to BCL2
Item Information
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Description
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
Product Overview
Entrez GenelD
596
Aliases
Bcl-2; PPP1R50
Clone#
7F9B12
Host / Isotype
Mouse / IgG2b
Immunogen
Purified recombinant fragment of human BCL2 (AA: 1-239) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Molecules. 2019 Oct 29;24(21):3896. 2.PLoS One. 2019 Oct 23;14(10):e0224247.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using BCL2 mAb against human BCL2 (AA: 1-239) recombinant protein. (Expected MW is 29.2 kDa)
Western Blot
Figure 3:Western blot analysis using BCL2 mAb against HEK293-6e (1) and BCL2 (AA: 1-239)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of Raji cells using BCL2 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.