BCL-2 Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma.Tissue specificity: Expressed in a variety of tissues.

Product Overview

Entrez GenelD

596

Aliases

Bcl-2; BCL2

Clone#

8E12

Host / Isotype

Mouse / IgG1

Species Reactivity

Human, Mouse

Immunogen

Synthetic peptide corresponding to residues surrounding BCL-2, conjugated to KLH.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1. J Biol Chem. 2010 Mar 26;285(13):9770-9.
2. Pharmacogenomics J. 2010 Feb 16.
3. J Int Med Res. 2009 Nov-Dec;37(6):1868-76.

Product Image

Flow cytometric

Figure 1: Flow cytometric analysis of 3T3L1 cells using BCL-2 mouse mAb (green) and negative control (purple).

Immunofluorescence analysis

Figure 2: Immunofluorescence analysis of NIH/3T3 cells using BCL-2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemical analysis

Figure 3: Immunohistochemical analysis of paraffin-embedded colon cancer tissues (left) and human brain tissues (right) using BCL-2 mouse mAb with DAB staining.

For Research Use Only. Not for use in diagnostic procedures.