BCAT2 Primary Antibody
Item Information
Catalog #
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Price
Description
This gene encodes a branched chain aminotransferase found in mitochondria. The encoded protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. Multiple transcript variants encoding different isoforms have been found for this gene.
Product Overview
Entrez GenelD
587
Aliases
BCAM; BCT2; PP18; BCATM
Clone#
7G3A11
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human BCAT2 (AA: 259-393) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Neurochem. 2012 Dec;123(6):997-1009.
2.Biochemistry. 2009 Jan 27;48(3):645-56.
2.Biochemistry. 2009 Jan 27;48(3):645-56.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using BCAT2 mAb against human BCAT2 (AA: 259-393) recombinant protein. (Expected MW is 41.5 kDa)
Western Blot
Figure 3:Western blot analysis using BCAT2 mAb against HEK293 (1) and BCAT2 (AA: 259-393)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of HeLa cells using BCAT2 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.