BAX Primary Antibody

Item Information
Catalog # Size/Concentration Price
32014 100ug $341.00
Specification
AliasesBCL2L4
Clone#6H8E11
Entrez GeneID581
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeMouse IgG2b
ImmunogenPurified recombinant fragment of human BAX(AA: (13-160)) expressed in E. Coli.
MW21.2kDa
Application
ELISA1/10000
IHC_P (Immunohistochemistry) 1/200-1/1000
FCM (Flow Cytometry)1/200-1/400
WB (Western Blot)1/500 - 1/2000
Sequence
AA: 13-160
Catalog
32014
$341.00
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Images

ELISA

Cell Culture Products
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

WESTERN BLOT

Cell Culture Products
Figure 2: Western blot analysis using BAX mAb against human BAX (AA:(13-160)) recombinant protein. (Expected MW is 36.3 kDa)

WESTERN BLOT

Cell Culture Products
Figure 3: Western blot analysis using BAX mouse mAb against Hela (1), C2C12 (2),C6 (3),HepG2 (4),MCF-7 (5),Ramos (6),Raji (7),HEK293 (8), and HEK293-6e (9) cell lysate.

FLOW CYTOMETRY

Cell Culture Products
Figure 4: Flow cytometric analysis of Hela cells using BAX mouse mAb (green) and negative control (red).

FLOW CYTOMETRY

Cell Culture Products
Figure 5: Flow cytometric analysis of Hepg2 cells using BAX mouse mAb (green) and negative control (red).

FLOW CYTOMETRY

Cell Culture Products
Figure 6: Flow cytometric analysis of Jurkat cells using BAX mouse mAb (green) and negative control (red).

IMMUNOHISTOCHEMISTRY

Cell Culture Products
Figure 7: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using BAX mouse mAb with DAB staining.

IMMUNOHISTOCHEMISTRY

Cell Culture Products
Figure 8: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using BAX mouse mAb with DAB staining.
Product Overview
Description

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. The association and the ratio of BAX to BCL2 also determines survival or death of a cell following an apoptotic stimulus. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Dec 2019]

References (references)
References (references) 1,J Biol Chem. 2019 Dec 13;294(50):19055-19065.2,Biochem Biophys Res Commun. 2019 Jun 30;514(3):881-887.

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