BAG1 Primary Antibody
Item Information
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Price
Description
The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. Multiple protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) initiation codon, and three alternative downstream AUG initiation codons. A related pseudogene has been defined on chromosome X.
Product Overview
Entrez GenelD
573
Aliases
HAP; BAG-1; RAP46
Clone#
2F7A11
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human BAG1 (AA: 219-346) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Oncol Rep. 2014 Oct;32(4):1441-6.
2.Cell Physiol Biochem. 2014;33(2):365-74.
2.Cell Physiol Biochem. 2014;33(2):365-74.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using BAG1 mAb against human BAG1 (AA: 219-346) recombinant protein. (Expected MW is 40.6 kDa)
Western Blot
Figure 3:Western blot analysis using BAG1 mAb against HEK293 (1) and BAG1 (AA: 219-346)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of HeLa cells using BAG1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.