B3GAT1 Primary Antibody

Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized.
Product Overview
Entrez GenelD
27087
Aliases
NK1; CD57; HNK1; LEU7; NK-1; GLCATP; GLCUATP
Clone#
3B8B10
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human B3GAT1 (AA: 193-334) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Biomed Res Int. 2014;2014:356427.
2.PLoS One. 2013;8(2):e52144.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using B3GAT1 mAb against human B3GAT1 (AA: 193-334) recombinant protein. (Expected MW is 41.5 kDa)
Figure 2:Western blot analysis using B3GAT1 mAb against human B3GAT1 (AA: 193-334) recombinant protein. (Expected MW is 41.5 kDa)
Western Blot
Figure 3:Western blot analysis using B3GAT1 mAb against HEK293 (1) and B3GAT1 (AA: 193-334)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using B3GAT1 mAb against HEK293 (1) and B3GAT1 (AA: 193-334)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using B3GAT1 mouse mAb against MOLT4 (1), Raji (2), and Ramos (3) cell lysate.
Figure 4:Western blot analysis using B3GAT1 mouse mAb against MOLT4 (1), Raji (2), and Ramos (3) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of Hela cells using B3GAT1 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of Hela cells using B3GAT1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.