B2M Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
ProductOrderB
Clone#4G5A1
Entrez GenelD567
FormulationPurified antibody in PBS with 0.05% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human B2M (AA: 21-100) expressed in E. Coli.
MW13.7kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
21-100
Catalog#: 30600
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Images
Western Blot
Figure 1: Western blot analysis using B2M mAb against human B2M (AA: 21-100) recombinant protein. (Expected MW is 35.4 kDa)
Figure 1: Western blot analysis using B2M mAb against human B2M (AA: 21-100) recombinant protein. (Expected MW is 35.4 kDa)
Western Blot
Figure 2: Western blot analysis using B2M mAb against HEK293 (1) and B2M (AA: 21-100)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using B2M mAb against HEK293 (1) and B2M (AA: 21-100)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of Hela cells using B2M mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)
Figure 3: Immunofluorescence analysis of Hela cells using B2M mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 4: Flow cytometric analysis of A431 cells using B2M mouse mAb (green) and negative control (red).
Figure 4: Flow cytometric analysis of A431 cells using B2M mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using B2M mouse mAb with DAB staining.
Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using B2M mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using B2M mouse mAb with DAB staining.
Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using B2M mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
DescriptionThis gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.
References (references)
References (references)1. Cancer Immunol Immunother. 2012 Sep;61(9):1359-71.
2. Lupus. 2012 Sep;21(10):1098-104.