AUP1 Primary Antibody

Item Information
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Size
Price
Description

The protein encoded this gene is involved in several pathways including quality control of misfolded proteins in the endoplasmic reticulum and lipid droplet accumulation. Lipid droplets are organelles in the cytoplasm that store neutral lipids such as cholesterol esters and trigylycerides to prevent the overabundance of free cholesterol and fatty acids in cells, but also to act as storage for other metabolic processes, such as membrane biogenesis. Reduced expression of this gene results in reduced lipid droplet clustering, a function that is dependent on ubiquitination of the protein. This protein contains multiple domains including a hydrophobic N-terminal domain, an acetyltranferase domain, a ubiquitin-binding CUE domain, and a UBE2B2-binding domain (G2BR). Alternative splicing results in multiple transcript variants.

Product Overview
Entrez GenelD
550
Clone#
1H6A1
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human, Mouse
Immunogen
Purified recombinant fragment of human AUP1 (AA: 229-410) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ELISA
1/10000
References
1.J Lipid Res. 2013 Feb;54(2):503-13.2.J Biol Chem. 2011 Feb 18;286(7):5599-606.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using AUP1 mAb against human AUP1 (AA: 229-410) recombinant protein. (Expected MW is 46.1 kDa)
Figure 2:Western blot analysis using AUP1 mAb against human AUP1 (AA: 229-410) recombinant protein. (Expected MW is 46.1 kDa)
Western Blot
Figure 3:Western blot analysis using AUP1 mAb against HEK293 (1) and AUP1 (AA: 229-410)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using AUP1 mAb against HEK293 (1) and AUP1 (AA: 229-410)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using AUP1 mouse mAb against A431 (1), NIH/3T3 (2), Hela (3), SW480 (4), CHO3D10 (5), A549 (6), and SPC-A-1 (7) cell lysate.
Figure 4:Western blot analysis using AUP1 mouse mAb against A431 (1), NIH/3T3 (2), Hela (3), SW480 (4), CHO3D10 (5), A549 (6), and SPC-A-1 (7) cell lysate.
For Research Use Only. Not for use in diagnostic procedures.