ATPIF1 Primary Antibody

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Description
This gene encodes a mitochondrial ATPase inhibitor. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified.
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using ATPIF1 mAb against human ATPIF1 (AA: 1-106) recombinant protein. (Expected MW is 38.2 kDa)
Figure 2:Western blot analysis using ATPIF1 mAb against human ATPIF1 (AA: 1-106) recombinant protein. (Expected MW is 38.2 kDa)
Western Blot
Figure 3:Western blot analysis using ATPIF1 mAb against HEK293 (1) and ATPIF1 (AA: 1-106)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using ATPIF1 mAb against HEK293 (1) and ATPIF1 (AA: 1-106)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using ATPIF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of Hela cells using ATPIF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 5:Flow cytometric analysis of Hela cells using ATPIF1 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of Hela cells using ATPIF1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using ATPIF1 mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using ATPIF1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ATPIF1 mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ATPIF1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.