ATP6V0A4

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Description

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. This gene is one of four genes in man and mouse that encode different isoforms of the a subunit. Alternatively spliced transcript variants encoding the same protein have been described. Mutations in this gene are associated with renal tubular acidosis associated with preserved hearing.

Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using ATP6V0A4 mAb against human ATP6V0A4 (AA: 228-390) recombinant protein. (Expected MW is 44.5 kDa)
Figure 2:Western blot analysis using ATP6V0A4 mAb against human ATP6V0A4 (AA: 228-390) recombinant protein. (Expected MW is 44.5 kDa)
Western Blot
Figure 3:Western blot analysis using ATP6V0A4 mAb against HEK293-6e (1) and ATP6V0A4 (AA: 228-390)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using ATP6V0A4 mAb against HEK293-6e (1) and ATP6V0A4 (AA: 228-390)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunofluorescence analysis
Figure 4:Flow cytometric analysis of Hela cells using ATP6V0A4 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of Hela cells using ATP6V0A4 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.