Mouse Monoclonal Antibody to ATP6AP1
Item Information
Catalog #
Size
Price
Description
This gene encodes a component of a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. Vacuolar ATPase (V-ATPase) is comprised of a cytosolic V1 (site of the ATP catalytic site) and a transmembrane V0 domain. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. The encoded protein of this gene may assist in the V-ATPase-mediated acidification of neuroendocrine secretory granules. This protein may also play a role in early development.
Product Overview
Entrez GenelD
537
Aliases
16A; CF2; Ac45; XAP3; XAP-3; ATP6S1; VATPS1; ATP6IP1
Clone#
5G12A4
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human ATP6AP1 (AA: 51-151) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Histol Histopathol. 2007 Apr;22(4):443-54. 2,Eur J Med Genet. 2020 Jun;63(6):103882.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/7db22c0f04a6455fc6362b3168b542cc2cfc8efb46477f714d1d5c4a5f5d4e80.jpg?v=1666705878)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using ATP6AP1 mAb against human ATP6AP1 (AA: 51-151) recombinant protein. (Expected MW is 37kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/39cafac1493d944afb4fc91a42ae59f073c119445182d7a5d639deb8c4eb6ac9.jpg?v=1666705878)
Figure 2:Western blot analysis using ATP6AP1 mAb against human ATP6AP1 (AA: 51-151) recombinant protein. (Expected MW is 37kDa)
Western Blot
![Figure 3:Western blot analysis using ATP6AP1 mAb against HEK293-6e (1) and human ATP6AP1 (AA: 51-151)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/69afb26b26596f08ada025da01c6333bf9334adf82be7d3aff0accf2be230f62.jpg?v=1666705878)
Figure 3:Western blot analysis using ATP6AP1 mAb against HEK293-6e (1) and human ATP6AP1 (AA: 51-151)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
![Figure 4:Flow cytometric analysis of Hela cells using ATP6AP1 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/46f69552aee44ab65daac2c4e12a1d7c45d02ae540eaad9720dfe073172a7eb9.jpg?v=1666705878)
Figure 4:Flow cytometric analysis of Hela cells using ATP6AP1 mouse mAb (green) and negative control (red).
Western Blot
![Figure 5:Western blot analysis using ATP6AP1 mouse mAb against PC-3 (1) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/b57a3d1a26a7b64313ba49eecc5e13fd6913c989557468317bd13411ae212604.jpg?v=1666705878)
Figure 5:Western blot analysis using ATP6AP1 mouse mAb against PC-3 (1) cell lysate.
For Research Use Only. Not for use in diagnostic procedures.