Mouse Monoclonal Antibody to ATP5F1A

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Description

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16.

Product Overview
Entrez GenelD
498
Aliases
OMR; ORM; ATPM; MOM2; ATP5A; hATP1; ATP5A1; MC5DN4; ATP5AL2; COXPD22; HEL-S-123m
Clone#
1A5B6
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human ATP5F1A (AA: 44-220) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Oxid Med Cell Longev. 2018 Nov 14;2018:1347174. 2.Oncol Rep. 2018 Feb;39(2):525-536.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using ATP5F1A mAb against human ATP5F1A (AA: 44-220) recombinant protein. (Expected MW is 44.7 kDa)
Figure 2:Western blot analysis using ATP5F1A mAb against human ATP5F1A (AA: 44-220) recombinant protein. (Expected MW is 44.7 kDa)
Western Blot
Figure 3:Western blot analysis using ATP5F1A mAb against HEK293-6e (1) and ATP5F1A (AA: 44-220)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using ATP5F1A mAb against HEK293-6e (1) and ATP5F1A (AA: 44-220)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using ATP5F1A mouse mAb against HepG2 (1), Hela (2), HCT116 (3), mouse heart (4), and rat heart (5) cell lysate.
Figure 4:Western blot analysis using ATP5F1A mouse mAb against HepG2 (1), Hela (2), HCT116 (3), mouse heart (4), and rat heart (5) cell lysate.
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ATP5F1A mouse mAb with DAB staining.
Figure 5:Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ATP5F1A mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using ATP5F1A mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using ATP5F1A mouse mAb with DAB staining.
Flow cytometric analysis
Figure 7:Flow cytometric analysis of Jurkat cells using ATP5F1A mouse mAb (green) and negative control (red).
Figure 7:Flow cytometric analysis of Jurkat cells using ATP5F1A mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.