Mouse Monoclonal Antibody to ATP1A1
The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/16db7a0fbf52c06095c4fdc2b8562d181478662b54c4d6adbaeca9d2f16ec45e.jpg?v=1666705619)
![Figure 2:Western blot analysis using ATP1A1 mAb against human ATP1A1 (AA: 153-288) recombinant protein. (Expected MW is 40.5 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/fa1442814e44ff1bab1c851029d6c9e49610a8a52bb1a5c6ceb4360c897c1c34.jpg?v=1666705619)
![Figure 3:Western blot analysis using ATP1A1 mAb against HEK293-6e (1) and ATP1A1 (AA: 153-288)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/87def8469780d3f2a3c464fb359c125dc24bc18495eab59c344c920fb4b812ce.jpg?v=1666705619)
![Figure 4:Western blot analysis using ATP1A1 mouse mAb against Hela (1) and A431 (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/5c32e5ea2aa197aa9b59357c225d2baae71c245eaf9a3a7dd362726f05e9a30a.jpg?v=1666705619)
![Figure 5:Immunofluorescence analysis of Hela cells using ATP1A1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/26f99f191c09b3e7aaceb724b9e9d4eb2e46e40ee3367d15120f1572280258e2.jpg?v=1666705619)
![Figure 6:Flow cytometric analysis of Hela cells using ATP1A1 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/74a2a2d5a3ae431000fcdeccaa7867e682cea7ad91d7899d0a1c3c25fe970a3a.jpg?v=1666705619)
![Figure 7:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using ATP1A1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/0c5fa0749fdfa7c00fc0db0b608df23a501aa6f197a1b19b088a7bde00df6df0.jpg?v=1666705619)
![Figure 8:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ATP1A1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/ca1d2eac525aaa0496fcb438207831dde67d9ca09c8db67b7d34d13dcf1ab504.jpg?v=1666705619)