Mouse Monoclonal Antibody to ATP1A1

Item Information
Catalog #
Size
Price
Description

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Overview
Entrez GenelD
476
Aliases
CMT2DD; HOMGSMR2
Clone#
3C1F3
Host / Isotype
Mouse / IgG2a
Immunogen
Purified recombinant fragment of human ATP1A1 (AA: 153-288) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Autophagy. 2018;14(8):1359-1375. 2.Sci Rep. 2016 Jun 14;6:27738.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using ATP1A1 mAb against human ATP1A1 (AA: 153-288) recombinant protein. (Expected MW is 40.5 kDa)
Figure 2:Western blot analysis using ATP1A1 mAb against human ATP1A1 (AA: 153-288) recombinant protein. (Expected MW is 40.5 kDa)
Western Blot
Figure 3:Western blot analysis using ATP1A1 mAb against HEK293-6e (1) and ATP1A1 (AA: 153-288)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using ATP1A1 mAb against HEK293-6e (1) and ATP1A1 (AA: 153-288)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using ATP1A1 mouse mAb against Hela (1), HepG2 (2), and A431 (3) cell lysate.
Figure 4:Western blot analysis using ATP1A1 mouse mAb against Hela (1), HepG2 (2), and A431 (3) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using ATP1A1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of Hela cells using ATP1A1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
Figure 6:Flow cytometric analysis of Hela cells using ATP1A1 mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of Hela cells using ATP1A1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using ATP1A1 mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using ATP1A1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ATP1A1 mouse mAb with DAB staining.
Figure 8:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ATP1A1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.