Mouse Monoclonal Antibody to ATP1A1
The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/08dbd38446659f6a252e7d7f1699e0f18abd0f404ae0f28eaa8b01628bde6107.jpg?v=1666705614)
![Figure 2:Western blot analysis using ATP1A1 mAb against human ATP1A1 (AA: 153-288) recombinant protein. (Expected MW is 40.5 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/c3514e981e2cc70db4efab57e93b9633b6a559a52c45710845f666c60325b55e.jpg?v=1666705614)
![Figure 3:Western blot analysis using ATP1A1 mAb against HEK293-6e (1) and ATP1A1 (AA: 153-288)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/d322883d3c6fdcab8ea7f1484a2fda83ca1193972a8c2cfeb56d384d68f0a3ee.jpg?v=1666705614)
![Figure 4:Western blot analysis using ATP1A1 mouse mAb against Hela (1), HepG2 (2), and A431 (3) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/89b2e31747765e2dbc1a7f6e1914c4a81335053bd3cf06d9e13adc447a8aadbf.jpg?v=1666705614)
![Figure 5:Immunofluorescence analysis of Hela cells using ATP1A1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/65ffeb86dad23c5f9cd7e981ffc5d09d1573f44e1d3e3ffff658a858483e5e1c.jpg?v=1666705614)
![Figure 6:Flow cytometric analysis of Hela cells using ATP1A1 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/12b5c8ec5b3ec8f77e3e8b6ad570a3191add8b3ec1afde5d9d7cc82e8207e762.jpg?v=1666705614)
![Figure 7:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using ATP1A1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/e2ef0de289de60d006addcc22c191abc9700503f909fb6345e2d0447557a9bad.jpg?v=1666705614)
![Figure 8:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ATP1A1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/3a39e7aa13f52a1ee7c93a9088bb58ef716a0752d25e87111292f8bde32fddc4.jpg?v=1666705614)