ATG5 Primary Antibody
The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene.
Purified antibody in PBS with 0.05% sodium azide
4°C; -20°C for long term storage
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using ATG5 mAb against human ATG5 (AA: 144-275) recombinant protein. (Expected MW is 41.5 kDa)
Figure 3:Western blot analysis using ATG5 mAb against HEK293 (1) and ATG5 (AA: 144-275)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using ATG5 mouse mAb against K562 (1), SH-SY5Y (2), and HCT116 (3) cell lysate.
Figure 5:Immunofluorescence analysis of Hela cells using ATG5 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Figure 6:Immunofluorescence analysis of Hela cells using ATG5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 7:Flow cytometric analysis of Hela cells using ATG5 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.