ATG4B Primary Antibody
Item Information
Catalog #
Size
Price
Description
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Product Overview
Entrez GenelD
23192
Aliases
APG4B; AUTL1
Clone#
6C3C5
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human ATG4B (AA: 1-221) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4?; -20? for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Oncotarget. 2016 Oct 11;7(41):66970-66988. 2.Blood. 2014 Jun 5;123(23):3622-34.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using ATG4B mAb against human ATG4B (AA: 1-221) recombinant protein. (Expected MW is 51 kDa)
Western Blot
Figure 3:Western blot analysis using ATG4B mAb against HEK293 (1) and ATG4B (AA: 1-221)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using ATG4B mouse mAb against Hela (1), RAW264.7 (2), Ramos (3), Jurkat (4), and HepG2 (5) cell lysate.
Flow Cytometric
Figure 5:Flow cytometric analysis of Hela cells using ATG4B mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.