ATG13 Primary Antibody
Item Information
Catalog #
Size
Price
Description
ATG13 (Autophagy Related 13) is a Protein Coding gene. Among its related pathways are Senescence and Autophagy and mTOR signaling pathway. GO annotations related to this gene include protein kinase binding.
Product Overview
Entrez GenelD
9776
Aliases
KIAA0652; PARATARG8
Clone#
3E4C8
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human ATG13 (AA: 339-550) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/100 - 1/500
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Mol Biol Cell. 2009 Apr;20(7):1992-2003.
2.Mol Cell Biol. 2009 Jan;29(1):157-71.
2.Mol Cell Biol. 2009 Jan;29(1):157-71.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using ATG13 mAb against human ATG13 (AA: 339-550) recombinant protein. (Expected MW is 48.9 kDa)
Western Blot
Figure 3:Western blot analysis using ATG13 mAb against HEK293 (1) and ATG13 (AA: 339-550)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using ATG13 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 5:Flow cytometric analysis of Hela cells using ATG13 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.