ATF3 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesN
ProductOrderA
Clone#7F1B10
Entrez GenelD467
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human ATF3 (AA: 1-181) expressed in E. Coli.
MW20.6kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
FULL(1-181)
Catalog#: 30864
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Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using ATF3 mAb against human ATF3 (AA: 1-181) recombinant protein. (Expected MW is 46.5 kDa)
Figure 2:Western blot analysis using ATF3 mAb against human ATF3 (AA: 1-181) recombinant protein. (Expected MW is 46.5 kDa)
Western Blot
Figure 3:Western blot analysis using ATF3 mAb against HEK293 (1) and ATF3 (AA: 1-181)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using ATF3 mAb against HEK293 (1) and ATF3 (AA: 1-181)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of MCF-7 cells using ATF3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of MCF-7 cells using ATF3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 6:Flow cytometric analysis of A549 cells using ATF3 mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of A549 cells using ATF3 mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 6:Immunofluorescence analysis of HeLa cells using ATF3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 6:Immunofluorescence analysis of HeLa cells using ATF3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Product Overview
DescriptionThis gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes.
References (references)
References (references)1.Arch Biochem Biophys. 2014 Dec 15;564:203-10.
2.Tumour Biol. 2014 Aug;35(8):8329-34.