ASF1A Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair.
Product Overview
Entrez GenelD
25842
Aliases
CIA; CGI-98; HSPC146
Clone#
2A5F8
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human ASF1A (AA: 1-204) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Science. 2014 Aug 15;345(6198):822-5.
2.PLoS One. 2012;7(1):e27759.
2.PLoS One. 2012;7(1):e27759.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using ASF1A mAb against human ASF1A (AA: 1-204) recombinant protein. (Expected MW is 48.9 kDa)
Western Blot
Figure 3:Western blot analysis using ASF1A mAb against HEK293 (1) and ASF1A (AA: 1-204)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of Hela cells using ASF1A mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.