AP2M1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Three transcript variants encoding different isoforms have been found for this gene.
Product Overview
Entrez GenelD
1173
Aliases
mu2; AP50; CLAPM1
Clone#
4F1B2
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Monkey
Immunogen
Purified recombinant fragment of human AP2M1 (AA: 298-435) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/300 - 1/800
ELISA
1/10000
References
1.PLoS Pathog. 2012;8(8):e1002845.
2.Proc Natl Acad Sci U S A. 2007 Feb 20;104(8):2991-6.
2.Proc Natl Acad Sci U S A. 2007 Feb 20;104(8):2991-6.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using AP2M1 mAb against human AP2M1 (AA: 298-435) recombinant protein. (Expected MW is 41.8 kDa)
Western Blot
Figure 3:Western blot analysis using AP2M1 mAb against HEK293 (1) and AP2M1 (AA: 298-435)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using AP2M1 mouse mAb against COS7 (1), SK-Br-3 (2), MCF-7 (3), T47D (4), and HEK293 (5) cell lysate.
For Research Use Only. Not for use in diagnostic procedures.