AOF1 Primary Antibody
| Item Information | ||
|---|---|---|
| Catalog # | Size/Concentration | Price |
| Specification | |
|---|---|
| Aliases | KDM1B; LSD2; C6orf193; bA204B7.3; dJ298J15.2 |
| ProductOrder | A |
| Clone# | 5E10C1 |
| Entrez GenelD | 221656 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Purified recombinant fragment of human AOF1 (AA: 6-129) expressed in E. Coli. |
| MW | 92kDa |
| Shipping Information | This product will ship in a box containing blue ice at a temperature of 4°C. Learn More |
| Species Reactivity | Human |
| Application | |
|---|---|
| ELISA | 1/10000 |
| ICC (Immunocytochemistry) | 1/200 - 1/1000 |
| IHC_P(Immunohistochemistry) | 1/200 - 1/1000 |
| FCM (Flow Cytometry) | 1/200 - 1/400 |
| WB (Western Blot) | 1/500 - 1/2000 |
| Sequence | |
|---|---|
| 6-129 | |
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using AOF1 mAb against human AOF1 (AA: 6-129) recombinant protein. (Expected MW is 40 kDa)
Western Blot
Figure 3:Western blot analysis using AOF1 mAb against HEK293 (1) and AOF1 (AA: 6-129)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of HeLa cells using AOF1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of HeLa cells using AOF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using AOF1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using AOF1 mouse mAb with DAB staining.
Flow cytometric
Figure 8:Flow cytometric analysis of HeLa cells using AOF1 mouse mAb (green) and negative control (red).